Immobilization of Plant Esterase Purified from Wheat Flour on Porous Poly (GMA-AM-EGDMA) Polymer Particles Newly Made With Liquid and Solid Pore-Forming Reagents

The Poly (GMA-AM-EGDMA) macrospheres were synthesized with glycidlymethacrylate (GMA), acrylamide (AM) as monomer and glycol dimethacrylate (EGDMA) as cross-linker, firstly with carbinol and distilled water as liquid pore-forming reagents and nano-calcium carbonate as solid one via suspension polymerization. Its exterior form was shown by scanning electron microscopy. Under the most suitable terms, the plant esterase isolated and purified from wheat flour was bounded on the particles above, the activity yield was 65.78% and the activity of it could reach 33.09 U/g dry carriers. Kinetic studies showed the pH operating range of the immobilized enzyme was wider and its thermal stability was better than those of the free enzyme, meanwhile, a good operational stability was also observed after being emploied 6 times repeatedly.